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Two experiments were conducted to examine the effects of growth regulator application on cytokinin levels in developing apple leaves. In experiment 1, gibberellin GA 4/7  (100 mg·liter -1 ) and benzyladenine (BA, 100 mg·liter -1 ) were applied alone or in combination as a foliar spray to `Golden Delicious' 7 days after full bloom. In experiment 2, BA was applied at 50 or 100 mg·liter -1  as a foliar spray to `Morespur McIntosh' 18 days after full bloom.  Trans -zeatin ribosidelike ( t -ZR) levels were determined by immunoassay. In experiment 1, GA 1/7  had no effect and BA increased  t -ZR-like levels. In experiment 2, BA increased  t -ZR levels quadratically with application rate. The increase in endogenous cytokinin levels with BA treatment occured within 2 hours. Initially,  t -ZR-like levels were 47 times higher in the BA (100 mg·liter -1 ) treated leaves as compared to check leaves. Over the next 8 days,  t -ZR-like content in treated leaves decreased in a cubic manner.

FOLIAR APPLICATION OF BENZYLADENINE INCREASES ENDOGENOUS LEAF CYTOKININ IN APPLE