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Seeds of `Burpless Hybrid' cucumber  (Cucumis sativus  L.) were separated from their seedcoats and excised into pieces comprising an embryonic axis and two cotyledons. The seed pieces were disinfested and explanted on modified Murashige and Skoog medium with 16 combinations of BAP (2.0, 3.0, 4.0, 5.0 mg·liter -1 ) and NAA (0.0,0.1,0.2,0.3 mg·liter -1 ). On all media, embryonic axes germinated and grew into whole plants within 4 weeks, but did not flower. Cotyledons developed adventitious shoots and male flowers on most media. A few female flowers developed. The best shoot regeneration and the most female flowers were observed on medium with 2.0 mg BAP and 0.3 mg NAA/liter. One of the female flowers was pollinated in vitro and it developed a small fruit with viable seed.

Regeneration and Flowering in Vitro of `Burpless Hybrid' Cucumber Cultured from Excised Seed