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Micropropagation Efficiency of Banana cv Kepok Amorang through Modifications of Culture Media and Incubation Temperature. Yati Supriati. The budless banana cv Kepok Amorang is potentially commercialized due to its sweet taste and does not have flower bud, hence reduced the potential of being infected by the blood disease pathogen. Enhancement of banana industry needs continuous supplies of large number banana seedlings. In vitro culture enable the production of seedlings in a large scale, uniform, quick. The research aims: (1) to formulate an efficient medium for in vitro multiplication of cv Kepok Amorang shoot, (2) to identify efficient growth environment for in vitro culture of cv Kepok Amorang, and (3) to formulate an efficient culture medium for roots inductions of cv Kepok Amorang. The plant material used was in vitro culture of Kepok cv Amorang, 2 cm in height without leaf and root. The media formulation for shoot multiplication were full strength, half strength, one fourth strength MS media, supplemented with either 1, 3, or 5 ppm IBA. On optimization step, the media tested were MS, Knop, Knop and Heller, Hyponex N, Growmore N, and Rosasol N containing of 1 ppm BA. The explants were incubated in culture room with 8, 12, and 16 hours photoperiod with temperatures 30oC (non air conditioned) and 25oC (air conditioned). The root induction trial was done using MS, Knop, Knop and Heller, Hyponex N, Growmore N, and Rosasol N media containing of 1 ppm and 3 ppm IBA. The results showed that the best medium formula for shoot multiplication was ¼ MS + 1 ppm IBA. The best incubation condition was 16 hours photoperiods at 30oC. The best media for root induction was Hyponex 2 g/l + 1 ppm IBA. This culture method reduced cost by Rp 261.7 per plantlet through efficiency of media formulation and electricity use.

Efisiensi Mikropropagasi Pisang Kepok Amorang melalui Modifikasi Formula Media dan Temperatur