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Isolation and Characterization of Actinomycetes Producing Antibacterial Compound into Enteropatogenik Escherichia coli K1.1, Pseudomonas pseudomallei 02 05 and Listeria monocytogenes 5407. Dwi N. Susilowati, Ratih D. Hastuti, and Erny Yuniarti. The resistance of bacterial pathogens to some antibacterial agents and side effects of the antibacterial usage demanded discovery of new effective, safe, and active antibacterial compounds. Some pathogenic bacteria, such as enteropathogen Escherichia coli (EPEC) that cause diarrhoea on children and infants, Pseudomonas pseudomallei that cause melioidosis on human and animal, and Listeria monocytogenes that cause listeriosis on newly born babies mortality and death of pregnant woman. Actinomycetes is the largest bacterial group that produce antibiotics. More than 10,000 antibacterial compounds had been discovered, two-third of them were produced by this bacterial group. A study was done to isolate and characterize Actinomycetes producing antibacterial compounds effective against EPEC K1.1 and P. pseudomallei 02 05. Soil samples were taken from 39 locations in Indonesia and 115 actinomycetes isolates were obtained. Two of the isolates, i.e., isolate A3.5 that was effective against P. pseudomallei 02 05 and isolate F6.1 that was effective against EPEC K1.1 evaluated further. The isolate A3.5 had an optimum time 72 hours to produce antibacterial compound, while F6.1 took 96 hours. The antibacterial compounds produced by both isolates were dissolve in the a 70% ethyl acetate solution, but not in a 40oC warm methanol solution because it is very dissolved. The antibacterial compound extracted from the isolate A3.5 had a similar effectiveness to antibiotics bacithracyn 10 unit and neomycin 30 g. On the other hand, the antibacterial compound extracted from isolate F6.1 had a similar effectiveness to antibiotics colistin 10 g and doxyciclin 30 g. Further identification of the isolates suggested that both of them belongs to the genera Streptomyces.

jurnal agrobiogen/jurnal agrobiogen

Isolasi dan Karakterisasi Aktinomisetes Penghasil Antibakteri Enteropatogen Escherichia coli K1.1, Pseudomonas pseudomallei 02 05, dan Listeria monocytogenes 5407