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This study aims to determine the rate of formation of biogas, to test the potential for methane gas formation and accelerate the anaerobic fermentation process and increase the production of biogas. The study consisted of 3 treatments, i.e. cow feces without the addition of inoculum, cow feces with the addition of rumen fluid waste by 25% and cow feces with the addition of mud peat by 25%. Each treatment consisted of 3 replications. Observations was done on the 10 th , the 20 th , 30 th and 40 th . Observations at the end of the fermentation test were conducted on methane. The obtained data were analyzed using split plot analysis calculations for the observation of biogas volume measurements, the concentration of methane gas, sludge digester temperature observations and the degree of acidity (pH). The result showed that pH and temperature were not significant. The volume of biogas showed significant results (P<0.05) having mean values of 228.67 ml with no inoculums, 229.65 ml for rumen fluid inoculums, 358.25 ml for peat mud inoculums with increasing values until 40 days. The concentration of methane showed significant results (P<0.05) having mean values of 35.91% with no inoculums, 35.74% for rumen fluid inoculums, 38.52% for peat mud inoculums with increasing values until 40 th days. The production of methane showed significant results (P<0.05) having mean values of 91.15 ml with no inoculums, 119.36 ml for rumen fluid inoculums, 150.62 ml for peat mud inoculums with increasing values until 40 th days. From the results of research it could be concluded that the addition of rumen fluid waste and sludge peat mud up to 25% could accelerate the process of fermentation. Further research is needed for a longer the fermentation process in fermentor to determine the maximum rate of production of biogas.

PENGARUH PENGGUNAAN LIMBAH CAIRAN RUMEN DAN LUMPUR GAMBUT SEBAGAI STARTER DALAM PROSES FERMENTASI METANOGENIK