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The aim of this research was to isolate and indentify the terpenoid compound from  Curcuma xanthorrhiza  rhizomes and its antibacterial activity. Isolation was carried out by using vacuum liquid chromatography and centrifugal chromatography. The structure was determined by NMR spectroscopy ( 1 H-NMR,  13 C-NMR 1D and 2D), then compare with data from literatures. Antibacterial test was carried out by using  microdillution methods and evaluated against eight bacteria. They are  Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Enterobacter aerogenes ,  Pseudomonas aeruginosa, Salmonella typhi ,  Shigella dysentriae  and  Vibrio cholerae . The result showed that the isolate was a white crystal which was indetified as germacron-type sesquiterpene. Germacron have highest activity againts  P. aeruginosa,  MIC   15.6 µg/mL  and  MBC 31.2 µg/mL.

Antibacterial Activity of Germacrone Sesquiterpene from Curcuma Xanthorrhiza Rhizomes