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Cucurbitane derivative has been isolated from methanol extract of the leaves of bitter melon (Momordica charantia). Purification of methanol extract using vacuum column chromatography method using n-hexane: ethyl acetate as the mobile phase was obtained a white powder isolate. This isolate was elucidated to obtain chemical structures based on spectroscopic data (FT-NMR, FT-IR, and LC-MS/MS) and resulted a cucurbitane derivate, namely momordicine I. The MeOH extract and the isolate were evaluated for α-glucosidase inhibitory effect. Both MeOH extract and momordicine I showed moderate activities with %inhibition 27.34% and 15.79 % at 100 μg/mL repectively. Keyword : Cucubitane, Momordica charantia, momordicine I, α-glucosidase INTRODUCTION Bitter melon (Momordica charantia) is a member of the Cucurbitaceae family which is easily cultivated and spreads to include China, India, and Southeast Asia. In Indonesia, bitter melon is consumed as a vegetable and used as traditional medicine. Utilization of M. charantia empirically carried out by the community is a cure for diabetes, heart disease and stomachache (Grover and Yadan, 2002). M. charantia has various medicinal potentials as antidiabetic (Joseph and Jini, 2013), antibacterial (Kumar et al., 2010) and antimicrobial (Svobodova et al., 2010). The antidiabetic effect is inseparable from the role of secondary metabolites compounds in the form of triterpenes, proteins, steroids, alkaloids, lipids, and phenolic compounds (Zhanga et al., 2012). M. charantia leaves contain momordica, momordine, charantine, trichosanic acid, resin, resinic acid, saponin, vitamins A and C (Tan et al., 2008). Previous studies have shown the compounds momordicoside A and M from M. charantia fruit have inhibitory activity Indonesia Chimica Acta Pamenta, et.al. p-ISSN 2085-014X Vol.12. No.2, December 2019 e-ISSN 2655-6049 100 against rat intestinal α-glucosidase enzymes (Nguyen et al., 2010). Earlier antidiabetic compound, momordicinin, has α-amylase inhibitory activity (Kulkarni et al., 2019). While in this study used α-glucosidase enzyme from saccharomyces cerevisiae recombinant. The enzyme α-glucosidase plays an important role in the hydrolysis of complex carbohydrates into glucose which can be absorbed through the intestine. Inhibition of α-glucosidase work can reduce glucose absorption in patients with hyperglycemia (Ben et al., 2017). One of the α-glucosidase inhibiting agents is acarbose which has been reported to reduce the intestinal absorption of sugar in humans (Jenkins et al., 1981). The results of this previous study encouraged this research to explore metabolite compounds contained in M. charantia leaves used methanol as extractor and tested their inhibitory activity against the α-glucosidase enzyme. MATERIALS AND METHODS

α-Glucosidase Inhibitory Activity Of Cucurbitane Derivate Isolated From Methanol Extract Of Momordica charantia L. Leaves