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Objectives: To evaluate the amount and type of microorganisms attached on tooth brushes with and without fluoride tooth paste and miswak after immediate brushing. Methods: A Cross sectional study was conducted on 29 randomly selected participants. They were instructed to brush their teeth once/week; with tooth brush alone, tooth brush with fluoride tooth paste and miswak alone. The samples were immediately processed for microbiological and scanning electron microscopic investigations. The frequency distribution, mean and standard deviation, were calculated the level of statistical significant difference was set at p ≤ 0.05. Results: All the participants cleaned their teeth with tooth brushes alone on the first day while 27 only used tooth brushes with fluoride tooth paste on the second week, whereas 24 participants used the miswak alone. The predominant type of bacteria revealed in the present study was Gram -ve/+ve cocci and Gram –ve/+ve bacilli. There was a direct correlation between tooth brushing methods used and type of bacteria. Friedman repeated measurement test revealed significant differences between the three brushing methods with p = .000 . The highest count of bacterial number was found in the miswak group and the lowest count in the tooth brush with fluoride tooth paste. Conclusion: The tooth brushes with or without fluoride tooth paste were carrying bacteria after each use including miswak. The tooth brushes should be thoroughly washed with sterile water and disinfected after each use. Additionally, the miswak used tip for brushing should be properly cut and trimmed after each single use.

Evaluation of the Amount and Type of Microorganisms in Tooth Brushes and Miswak after Immediate Brushing