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NEK5 is a member of the NEK kinase family that participates in cell cycle regulation, and studies have demonstated that NEK5 is a substrate for caspase-3 and is associated with cell death and cell respiration. To better evaluate the relation between this kinase and cell death, the aim of this project is to analyse the role of NEK5 in the apoptosis pathway. For this, the expression of proteins related to this pathway will be quantified by Western Blot in cells expressing NEK5 and in cells depleted for it, in tumor and non-tumor cell lines. The expression of proteins in non-tumor cell lines suggest that the expression of NEK5 is connected to an anti-apoptotic signaling in the cell.

Evaluation of the apoptosis pathway in tumor and non-tumor cell lines with altered expression of the NEK5 kinase