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Masculinization on tilapia ( Oreochromis sp.) has been done with approach of natural hormone steroid agent  from bee resin  through artificial diet before sexual differentiation. This research aim to increase male sex ratio and production performance. Treatment of bee resin dose is 0.6; 1.2; 1.8; 2.4; 3.0 feed ml/kg compared to Control (0 ml/kg diet) were given  during 28 days at larva age 7 day in aquarium, then transferred to the farm ground until age 12 weeks. Average of masculine phenotype percentage increased significantly (P Keywords: bee resin, masculinization, masculine sex ratio, Oreochromis sp. ABSTRAK Maskulinisasi pada ikan nila  (Oreochromis sp.) telah dilakukan dengan pendekatan hormonal menggunakan bahan aktif steroid alami yang berasal dari resin lebah melalui pakan buatan sebelum diferensiasi kelamin. Penelitian ini bertujuan untuk meningkatkan meliputi kelangsungan hidup, nisbah kelamin jantan, laju pertambahan bobot, dan konversi pakan (FCR).  Perlakuan yang diuji terdiri dari 5 dosis resin lebah  (0,6; 1,2; 1,8; 2,4; 3,0 mL/kg pakan) dan Kontrol, diberikan selama 28 hari pada larva berumur 7 hari di akuarium, kemudian ikan dipelihara di kolam tanah sampai umur 12 minggu. Rata-rata persentase fenotipe jantan meningkat secara nyata (P Kata kunci: Oreochromis sp, maskulinisasi, nisbah kelamin jantan, resin lebah.

Masculinization on tilapia (Oreochromis sp.) by natural hormone steroid agent from bee resin through artificial diet