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Fish requires essential fatty acid for growth. Freshwater fish needs linoleat fatty acid (n-6) or combination of linoleat and a-linolenat acids (n-3).  Fish oil contains higher level of n-3, corn oil is rich of n-6, while coconut oil is rich of saturated fatty acids.  This study was conducted to determine the effect of fatty acid sources in diet on growth performance of botia Botia macracanthus . Sources of fatty acid examined were coconut oil (control), corn oil, fish oil, and corn oil + fish oil + coconut oil. The results of study show that daily growth rate of fish fed on diet containing mix of corn-coconut-fish oils (8.39%) and only corn oil (8.15%) was higher (p 0.05).  Survival rate of fish at all treatments was similar, ranged from 90.00 to 93.33%.  Thus, the results suggested that diet containing only corn oil is suitable for botia to obtain higher growth rate and lower feed conversion rate. Keywords: fatty acid, growth performance, botia, Botia macracanthus ABSTRAK Ikan membutuhkan asam lemak essensial (EFA = Essential Fatty Acid) untuk pertumbuhannya.  Ikan air tawar memerlukan asam lemak linoleat ( n -6) atau gabungan asam lemak linoleat (n-6) dan alfa-linolenat (n-3).  Minyak ikan banyak mengandung asam lemak n -3, minyak jagung kaya akan asam lemak n-6, sementara minyak kelapa banyak mengandung asam lemak jenuh.  Sumber asam lemak yang diuji adalah minyak kelapa  (kontrol), minyak jagung, minyak ikan, dan minyak jagung + minyak ikan + minyak kelapa. Hasil penelitian menunjukkan bahwa laju pertumbuhan harian ikan botia yang diberi pakan yang mengandung campuran minyak jagung-kelapa-ikan (8,39%) dan minyak jagung (8,15%) saja lebih tinggi (p 0,05).  Kelangsungan hidup ikan pada semua perlakuan relatif sama, yaitu berkisar antara 90,00-93,33%. Dengan demikian, pakan untuk ikan botia cukup mengandung minyak jagung saja untuk mendapatkan laju pertumbuhan yang tinggi dengan konversi pakan yang rendah. Kata kunci: asam lemak, kinerja pertumbuhan, ikan botia, Botia macracanthus

Effect of Different Fatty Acid Sources of Diet on Growth Performance of Botia Botia macracanthus Bleeker