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The present study aims to isolate the fatty acid from pangasius fish oil by hydrolysis method and determine the factors that affect the enrichment of the high bioactive chemical elements such as Docosahexaenoic Acid (DHA) and Eicosapentaenoic Acid (EPA) which are two polyunsaturated fatty acids in Omega-3 group by urea complexation process. At the same time, the optimization determined the relationship between mathematics and technology to choose the best technological solution. The recovery efficiency of fish oil reached maximum value at the urea-to-fatty acid ratio of 3.2, complexation time of 16 hours and complexation temperature of -10.6°C. Under the optimal conditions, the enrichment ratio of DHA and EPA also reached maximum value of 75.5 and 78.9% in order to enhance the value of pangasius oil raw material and significantly improve the quality of final product.

Determine the Factors that Affect the Enrichment Process of High Bioactive Substance from Pangasius Oil