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The effects of treatment with various doses of carcinostatic agents on tumor growth have been studied. In animal experiments. treatment with low concentrations of nitrogen mustard N-oxide resulted in an early death of mh:e bearing Ehrlich ascites carcinoma and an increase in the number of circulating Yoshida sarcoma cells. The growth of subcutaneously implanted Yoshida sarcoma was stimulated after contact of the cells with Jow concentrations of nitrogen mustard N·oxide in vitro or intraperi­ toneally. Low concentrations of Mitomycin-C and Chromomycin As also showed increase of tumor growth and metastasis formation. In vitro study showed that the proliferation of HeLa cells was stimulated by adding low concentrations of nitrogen mustard N-oxide. Mitomycin-C, and Chromomycin As into the culture media. In clinical studies, much more cancer ceIls were released into the stomach cavity after the administration of nitrogen mustard N·oxide, Mitomycin-C, and Chromomycin As.

Production of metastases by treatment with carcinostatic agents. 3. Low concentration of carcinostatic agents on the cells.