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Configurational changes of the mitochondrial inner ·membrane system were examined ultrastructurally and biochemically. Mitochondria in intact heart muscle appeared predominantly in a so-called "orthodox" mode. On the other hand, the mitochondria isolated from liver or heart muscle showed a so-called "aggregated" mode. Many authors who have examined ex tensively isolated mitochondria found that they underwent ultrastructural changes coupled with their functional metabolic states, corresdonding to the surrounding conditions such as substra tes, Pi, nucleotides or uncouplers. Intact heart muscles were fixed by perfusion with glutaraldehyde solutions, and two types of configurational states of mitochondria were found. The first type of mitochondria showed an irregular and relatively straight ar rangement of the inner membrane system. The second had an angular and r egular configuration. Ehrlich ascites tumor cells also exhibited the twisting and waving configuration of mitochondrial inner membrane when fixed at the state of r espiratory inhibition induced by addition of glucose. Mitochondria of fasted ascites cells showed an irregular configuration. Mitochondria in the rough homogenates of rat heart muscle had morphologically· a transitional structure between isolated mitochondria and in situ ones. Mitochondria in the homogenates fixed at various functional metabolic states showed particular configura tional changes cor relating to their functional states in both "orthodox" and "aggr egated" modas. From in vitro experiments, the angular or twisted configuration of the inner m embrane of mitochondria was found to be induced by oxidizable substrates and Pi. As intact cells possess Pi endogenously, the mitochondria in situ are consider ed to have two different configurations. ilf\'i:llfii¥~ ¥Received for publication March, 12, 1971. * This work was in part presented at the 58th Genera l Meeting of the Japanese Pathological Society on April 3, 1969. * Abbrevia tions used: Pi, inorganic phosphate; DNP, 2, 4-dinitr ophenol; EDTA, ethyle, nediamine tetraacetate; ADP, adenosine 5'-diphosphate; ATP, adenosine 5'-triphosphate. ** Retired from Nagoya University School of Medicine March 31, 1969.

nagoya journal of medical science

Studies on the structure and function of biological membrane system. II. Configurational changes of mitochondria in situ correlating to functional states.