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BACKGROUND:  Rapid and accurate identification of  Staphylococcus capitis  is required to provide a better prognosis for endocarditis patients and tackle the emergence of multidrug resistant strains of the bacteria in hospitals. The current study was aimed to develop polymerase chain reaction (PCR) assay for specific identification of  S. capitis  using  sodA  and  gap  genes as markers.   METHODS:  Five sequences of  sodA  and sixteen sequences of  gap  registered in GeneBank were analysed using bioinformatic tools. PCR primers were designed based on the conserved and specific regions of  sodA  and  gap . Four clinical isolates of  S. capitis  (named no. 56-59) and six reference strains of coagulase-negative staphylococci (CoNS) species including  S. epidermidis  ATCC 35984,  S. epidermidis  48951I/09,  S. lugdunensis  44987/09,  S. sciuri  109645I/08,  S. warneri  135612/09,  S. hominis  114202/08 were used to validate the conventional PCR system.   RESULTS:  The current PCR system only amplified the DNA template of  S. capitis.  Current primers specifically targeted  S. capitis  as the agarose images only showed bands from  S. capitis  samples.   CONCLUSION:  The  sodA  and  gap  genes might serve as effective markers for identification of  S. capitis  using conventional PCR. The PCR assay in the current study was able to identify five clinical isolates of  S. capitis  accurately without mispriming, misamplification and misidentification. The PCR system was also able to discriminate other CoNS including  S. epidermidis ,  S. lugdunensis ,  S. sciuri ,  S. warneri  and  S. hominis .   KEYWORDS:  Staphylococcus, S. capitis, sodA, gap,  PCR

sodA and gap Genes as Markers for The Identification of Staphylococcus capitis