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In this manuscript we report development of the conductometric biosensor for the pesticides detection and analyze the possibility of its reactivation after inhibition. A differential pair of planar gold interdigitated electrodes deposited on the sytal substrate served as a transducer of the conductometric signal. Butyrylcholinesterase (BuChE) coimmobilized by glutaraldehyde crosslinking with bovine serum albumin on the trancducer surface was used as a bioselective element. The biosensor calibration curves were obtained for different concentrations of butyrylcholine (BuCh) and optimal concentration was found. Next, we tested the biosensor sensitivity to organophosphorus pesticide trichlorfon, the calibration curve was plotted. We showed the possibility of the bioselective membrane reactivation after irreversible enzyme inhibition, using the solution of pyridine-2-aldoximemethyliodide (PAM-2) as a reactivator. The conditions of reactivation of BuChE-based biomembranes, such as dependence on the duration of reactivation and the toxicant and reactivator concentrations, were investigated and optimized.

INVESTIGATION AND OPTIMIZATION OF REACTIVATION BUTYRYLCHOLINESTERASE-BASED BIOSENSOR FOR INHIBITORY ANALYSIS OF PESTICIDES