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This study was done in order to detect the hepatitis B virus (HBV) DNA copies in patients through blood transfusions; recessive carriers with HBsAg negative but HBV DNA positive were further studied to see the content and distribution of HBV in patients, and provide evidence for the clinical treatment. A total of 532 blood samples collected from July 2014 to July 2015 were tested for HBV-DNA viral load and hepatitis B serological markers using quantitative Polymerase Chain Reaction (qPCR) and serologic test (the five serological markers of hepatitis B). The results showed that, 3 cases were HBV serology negative and the HBV-DNA viral load was in the range of 250–500 whereas only 1 case was HBsAb positive and the HBV-DNA viral load was in the range of 500 and above. Quantitative PCR, for detecting HBV DNA, together with serological routine test can effectively reduce the risk of transfusion and prevent medical disputes.

Detection of HBV DNA by PCR and its application in clinical transfusion