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The aim of the study was to determine the level of sex steroid hormones in white matter of the brain of rats  with tumors combined with chronic neurogenic pain (CNP), which was modeled by bilateral sciatic nerve ligation.  The study included albino male rats (n=74). In the main group, M1 sarcoma was transplanted subcutaneously (n=11)  or into the subclavian vein (n=11) 45 days after CNP modeling. Two comparison groups (n=13 each) included  sham operated animals (without CNP) with M1 sarcoma transplanted subcutaneously and intravenously. Control groups  included animals with CNP and sham operated animals. Rats were euthanized on day 21 of the carcinogenesis.  Levels of total and free testosterone (T), estrone (E1), estradiol (E2), estriol (E3) and progesterone (P4) in the brain  white matter were measured using ELISA kits (“Cusabio”, China). CNP caused a decrease in the total and free T  by 1.5 times (p

Influence of malignant growth and chronic neurogenic pain on neurosteroid levels in rat brain