English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background: Cervical carcinoma is gynecologic malignancy with conventional treatment modality but drug resistance interferes with current therapeutic methods. Therefore, identification of novel new modality with low toxicity has uniquely favorable strategy. Objectives: The aim of this study is evaluation of concomitant use of sea cucumber organic extract and radiotherapy on ovarian cancer. Materials and Methods: In this in vitro experimental study, HeLa cancer cells were cultured and suspended in RPMI (Roswell Park Memorial Institute) 1640 medium supplemented with 10 % FBS (Fetal Bovine Serum), 1 % antibiotic. Cells were treated with extract at dierent concentrations (0 to 100 g/mL) for 24 hours. After determination of suitable concentration, the cells were exposed to 2 Gray gamma radiation in presence of extract for 192 seconds and 66 hours were kept in incubator till anti-proliferative assay were evaluated. To assess apoptosis, flow cytometry with PI (Propodium Iodide) and acridine orange staining were performed. Results: Morphological analysis and results from cytotoxicity assay exhibited that 50 g/mL of sea cucumber extract alone is con- sidered IC50 and combination of gamma radiation became more valuable in growth inhibition. Also, flow cytometry histogram of treated cells indicated sub-G1 peak demonstrating disturbance in membrane integrity and apoptosis cell death. Fluorescence images have been confirmed apoptosis cell death in treatment groups. Conclusions: These data indicate that sea cucumber extract as novel resource of aquatic natural products significantly can inhibit cervical cancer cell growth and synergistic eect of natural extract along with radiation therapy was more eective in anti-cervical

Concomitant Use of Sea Cucumber Organic Extract and Radiotherapy on Proliferation and Apoptosis of Cervical (HeLa) Cell Line