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Low intensity laser therapy has been used to accelerate the ulcers healing due to its healing, anti-inflammatory, analgesic and anti-edematous effects. Contaminations by bacteria or fungi on these ulcers commonly happen, constituting a major complication of healing, but there is no consensus about laser therapy bactericidal or fungicidal effect. Objective: To analyze the effects of different times of appliantion using the 808nm and 660nm lasers on cultures of different bacterial species and fungi in vitro. Method: Fifteen Petri plates were prepared, 12 plates containing Gram-positive (3 Staphylococcus aureus plates, 3 Enterococcus plates) and gram negative bacterias (3 Escherichia coli , 3 Klebsiella pneumonia plates) and 3 Petri plates with the fungi Candida albicans . Plates were randomly divided into 3 groups with 5 plates each group. Plates of Group 1 were irradiated with 660 nm laser at nine different points (A, A 1 , A 2 /B, B 1 , B 2 /C, C 1 , C 2 ), and points A/B/C reciebed only one application, points A 1 ,B 1 ,C 1 received 2 applications and points A 2 ,B 2 ,C 2 received 3 applications. Plates from group 2 where irradiated with 660nm also in nine diferente points, following the same criterea of group 1, while plate of group 3 where used as control, without irradiation. Groups 1 and 2 were irradiated with 144 J/cm² dose at point A (irradiation time of 2 minutes and 15 seconds), point B (irradiation time of 1 minute 7 seconds) and at point C (40 seconds). After the irradiations the plates were incubated for 24 hours. The cultures were visually examined to check the presence or not of the inhibition zone. Results: In all the groups, no zone of inhibition nor growth were observed, indicating no bactericidal/fungicidal and/or bactericidal effect, in other words, in all groups and locations with different time exposures (A - 30 mW, B - and C 60 mW - 100 mW) the growth of bacteria and fungi were equal. Conclusion: Low intensity laser therapy (808nm and 660nm) did not produce bactericidal and/or bacteriostatic effects, and even no bio-stimulant effect on the bacteria.

Analysis of low level laser therapy in vitro cultures of bacteria and fungi