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| The present study was conducted to evaluate the effect of insulin on blood level of estrodiol-17β (E2) and LH. The experiment was conducted using forty-eight cows and divided into Treatment (I, II and III) and control groups each consisting of 12 animals. Insulin was injected @0.25 IU/Kg body weight subcutaneously on day 0-3, 4-7 and 8-11 of estrous cycle in group I, II and III, respectively and PBS was injected in control animals. Blood samples were collected on day 0, 5, 10, 16 and 21 of estrous cycle. Serum was separated and stored at -20°C till analysis. In pregnant animals, E2 level in blood on day 5 was significantly (P<0.01) lower in group I compared to control and group III. For the same day it was significantly (P<0.05) lower in group II compared to group III and control. Serum concentration of LH, in pregnant animals on day 0, 5 and 10 was significantly (P<0.01) higher from day 21 in group III. Serum LH concentration on day 5 and 10 was also significantly (P<0.05) higher than day 16. These results indicated that insulin treatment declined the estrogen at day 5 and enhanced LH secretion up to day 10 reflecting its beneficial effect in corpus luteum survival and further conception.

Effect of Insulin on Post-AI Circulating Estrodiol-17β and LH in Crossbred Cattle