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Concentrating glucose after enzymatic hydrolysis of lignocellulosic biomass is an important step in order to prepare the stream to undergo the fermentation process. However, glucose itself can be inhibitor for the enzymatic hydrolysis, hence in situ glucose removal is recommended. Ultrafiltration represents a promising procedure for isolation of enzymes from hydrolysate and for the removal of glucose. One of the obstacles in successfully utilizing ultrafiltration’s membranes has been due to the membrane fouling. The flux behavior of polysulfone (PSF) membrane was studied in concentrating glucose from cellulose hydrolysate during dead end ultrafiltration. Different pH of solutions was used and Kumar’s model was applied to analyse the fouling mechanism. The minimum fouling was obtained at pH solution above the IEP due to protein-protein and membrane-protein repulsions alleviating aggregation and fouling. Cake formation blocking was identified as the dominant mechanism for flux decline.

EFFECT OF pH ON FLUX DECLINE DURING FRACTIONATION OF GLUCOSE FROM CELLULOSE HYDROLYSATE THROUGH A POLYSULFONE MEMBRANE