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Objectives: Classic Hodgkin lymphoma (CHL) is not typically diagnosed by routine clinical flow cytometry in most laboratories. While the neoplastic Hodgkin lymphoma/ Reed Sternberg (HRS) cells in CHL may be identified by flow cytometry using special procedures, the methodology used is not common. We observed increased CD38 expression on T-cells compared to Bcells/non-T-cells in cases of CHL compared to normal samples and confirmed our observations in the context of retrospective and prospective analyses. We identified and characterized distinctive features of background non-neoplastic T cells associated with classic Hodgkin lymphoma utilizing common antibodies and analysis methods.

Utility of T-cell activation patterns determined by flow cytometry for prediction of classic Hodgkin lymphoma