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In this paper, authors describe how to carry out a research assessing the impact of storage temperature on the stability and biocidal activity of formulated essential oils. Cereals, like other stored food, currently experience serious health problems of storage. The offending agents are primary pests such as Sitophilusoryzae and secondary pests including Tribolium. This study focused on the evaluation of the biocidal effect on the essential oils of thyme and citrus, against red flour beetle adults (Triboliumcastaneum) under different temperature regimes. The results showed that different molecules produced a delayed effect (12 h-14 h-16 h-18 h) on the populations of Triboliumcastaneum at the storage temperature of 20 °C. The same results showed an early striking effect of thymol and carvacrol on Triboliumcastaneum compared to limonene. The toxicity of thymol and carvacrol was much more active than limonene at 12 h, which was very remarkable as limonene expressed its toxicity from 14 h with a deadly effect. When temperatures are under 20 °C and 25 °C, thymol showed a greater degree of efficiency followed by carvacrol and limonene which had lower efficiency. However, at 28 °C, carvacrol expressed a very significant biocidal effect compared to the other two formulations, thymol and limonene.

journal of environmental science and engineering. a

Assessing the Impact of Storage Temperature on the Stability and Biocidal Activity of Essential oils Formulated. Case Tribolium Castaneum (herbst). (insecta, tenebrionidae)