English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Detection of Hepatitis C virus (HCV) RNA, the earliest marker and a direct indicator of ongoing viral replication, is more reliable than the anti HCV antibodies for screening for an ongoing HCV infection especially in high risk patients, such as those on hemodialysis (HD). Newer molecular methods that are affordable with a potential for point-of-care testing are being increasingly developed and recommended for an early and cost effective detection of HCV RNA. An in house developed HCV Reverse Transcriptase Loop mediated Isothermal Amplification (RT-LAMP) assay targeting the 5’ un-translated region (UTR) of the HCV genome, was performed simultaneously on Plasma and Dried blood spots (DBS) from a study group of 300 high-risk patients {250 hemodialysis (HD) and 50 Chronic Liver Disease (CLD)} and 50 healthy age matched individuals (control group). Among the study group, 145/300 (48.3%), (110/250 (44%) HD and 35/50 (70%) CLDs) were positive for HCV RNA on the DBS. The HCV RT-LAMP was 100% sensitive and specific with a % CV less than 10, as compared to the gold standard HCV Real Time PCR and a nested RT-PCR. The detection limit of all the assays was 50 copies of RNA /ml.

Application of Real Time Loop Mediated Isothermal Amplification Assay on Dried Blood Spots in the Detection of HCV RNA among High Risk Patients