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This work aimed to evaluate the viability of Lactobacillus acidophilus immobilized in calcium alginate sphere under different stress conditions. For the preparation of the sphere was dripped into a solution of 5 % (w/v) sodium alginate bacterial suspension of Lactobacillus acidophilus, previously activated. To simulate the acid stress, free and immobilized bacteria were exposed to pH values 2.5, 3.5 and 7.0, for 4 hours, incubated at 37 °C. The same was done for salt stress, at concentrations of 0.85 %, 4 % and 6 %. For freezing stress, the bacteria free and immobilized was stored at 20 °C for three months. In all simulations of stress conditions (pH, salts and freezing) changes in count for the immobilized bacteria were lower, with the greatest protection occurred when Lactobacillus acidophilus immobilized was subjected to high osmotic pressure in a concentration of 6 % NaCl and freezing. We can conclude calcium alginate acted as a protective agent in adverse conditions, providing protection to the microorganism. Therefore, the immobilization technique in calcium alginate has good prospects for the development of new probiotic foods.

Viability of Lactobacillus acidophilus Immobilized in Calcium Alginate Spheres and Submitted to Different Conditions of Stress