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Using feminizing adrenal neoplastic gland (FANG) cells we showed that hPRL stimulated cGMP biosynthesis. Also we could clearly demonstrate a stimulatory effect of db-cGMP plus theophylline on estrogen production without concomitant stimulation of cell growth. These findings indicate that a stimulatory effect of hPRL on estrogen biosynthesis by Fang-8 cells may act via increased cGMP concomitant with depressed cAMP.

A role of guanosine 3',5'-monophosphate in human-prolactin-induced estrogen production by feminizing adrenal neoplastic cells.