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Um metodo estavel, confiavel, eficiente com relacao a tempo e custo para deteccao de acucares redutores, baseado no metodo colorimetrico do acido dinitrossalicilico (DNS), adaptado para placas de microtitulacao em um banho de agua modificado com tratamento de microondas e proposto. Alem da influencia benefica sobre o meio ambiente devido a reducao do volume dos reagentes utilizados, o metodo modificado e rapido e permite a analise de um numero relativamente alto de amostras. Pode ser aplicado em diversos tipos de laboratorios, de processamento de alimentos a industrias farmaceuticas. O metodo adaptado foi parcialmente validado (intervalo de linearidade entre 7 µg e 5 mg per poco; limite de deteccao 3 µg per poco, desvio padrao em torno de 5% para curvas de calibracao com glucose, e recuperacao superior a 90%) e aplicado a series de produtos contendo acucares redutores, como amostras de mel e vinho e medicamentos que contem lactose como excipiente. O metodo proposto e bem adaptado como uma tecnica de alto rendimento para determinacao de acucares redutores, pois todas amostras analisadas deram resultados esperados de acordo com as suas notas tecnicas. A sound, reliable, time- and cost-efficient method for detection of reducing sugars, based on the well known dinitrosalicylic acid (DNS) colorimetric method, adapted for microtiter plates, in a modified water bath with microwave treatment is proposed. Besides the benefic influence on the environment due to the reduction of the volume of the reagents, the modified method is rapid and allows the analysis of a relatively high number of samples. It can be applied in many types of laboratories from food processing to pharmaceutical industries. The adapted method was partially validated (linearity range between 7 µg and 5 mg per well; limit of detection 3 µg per well, standard deviation around 5% for calibration curves with glucose, and a recovery higher than 90%) and applied to a series of products containing reducing sugars, such as honey, wine samples and drugs containing lactose as an excipient. The proposed method is well-suited as a high-throughput technique for determination of reducing sugars as all the analyzed samples gave the expected results according with their technical notes.

Adapting the reducing sugars method with dinitrosalicylic acid to microtiter plates and microwave heating