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Milk fatty acids profile was evaluated using12 Holstein cows producing 15 kg/day of milk with 4.0% of fat. The animals were assigned to four treatments in a 2 x 2 factorial arrangement (two tropical grasses -Brachiaria brizanthacv. Xaraes and Panicum maximum cv. Tanzania, supplemented with two concentrate levels - 3 and 6 kg/cow/day) in a completely randomized block design. There was no effect of forage and concentrate level (P>0.05) on concentrations of rumenic, vaccenic, (C18: 1 trans-11) and oleic acids in milk fat (mean values of 1.01 1.79, and 22.55 g/100 g of total fatty acids, respectively). The ,concentrations of lauric (C12:0), myristic (C14:0), and palmitic (C16:0) acids were also unaffected by treatments (mean values of 2.63, 9.77, and 27.44g/100g of total fatty acids, respectively). Overall, the results illustrate the potential of tropical grasses in producing milk containing a more desirable fatty acids profile in terms of human health. Keywords: dairy cow, conjugated linoleic acid, Brachiaria brizantha, Panicum maximum

Perfil de ácidos graxos no leite de vacas Holandês em pastagens tropicais suplementadas com dois níveis de concentrado