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The effect of co-culturing varying concentrations of pig and human red blood cells (RBCs) on the baseline frequency of sister chromatid exchanges (SCEs) and cell-cycle progression in pig plasma (PLCs) and whole blood leukocyte cultures (WBCs) was studied. No variation in SCE frequency was observed between pig control WBC and PLC. Addition of pig and human RBCs to pig PLCs did not modify the baseline frequency of SCEs. On the other hand, cell proliferation was slower in PLCs than in WBCs. The addition of pig or human RBCs to PLCs accelerated the cell-cycle progression of pig lymphocytes. When RBCs were added to PLCs the concentration and time sequence of RBC incorporation affected the cell-cycle progression of swine lymphocytes. When doses of pig or human RBCs equivalent to those present in WBCs were added immediately after PLC stimulation, the cell-cycle kinetics were similar to those of WBCs. Shorter co-incubation periods or a reduction in the dose of RBCs made cell-cycle progression intermediate between PLC and WBC values. Thus, pig and human RBCs modulated the in vitro cell-cycle progression of pig lymphocytes in a time- and dose-dependent manner, and the low baseline frequency of SCEs of pig lymphocytes is independent of the presence or absence of erythrocytes in culture Foi estudado o efeito de co-culturas com concentrações variadas de células sangüíneas vermelhas (RBCs) suínas e humanas na freqüência basal de trocas de cromátides irmãs (SCEs) e na progressão do ciclo celular em culturas de plasma de porco (PLCs) e culturas leucocitárias do sangue total (WBCs). Não foi observada nenhuma variação na freqüências de SCEs entre os controles de WBC e PLC em porcos. A adição de RBCs de suínos e humanos a PLCs não modificou a freqüência basal de SCEs. Por outro lado, a proliferação celular foi mais lenta em PLCs que em WBCs. A adição de RBCs humanas ou suínas a PLCs acelerou a progressão do ciclo celular de linfócitos suínos. Quando RBCs foram adicionadas a PLCs, a concentração e a seqüência temporal da incorporação de RBC afetaram a progressão do ciclo celular de linfócitos suínos. Quando adicionadas doses de RBCs suínas ou humanas equivalentes àquelas presentes em WBCs, imediatamente após estimulação da PLC, a cinética do ciclo celular foi similar à das WBCs. Períodos mais curtos de co-incubação ou uma redução na dose de RBCs causaram uma progressão do ciclo celular intermediária entre os valores de PLCs e WBCs. Assim, RBCs suínas e humanas modularam a progressão do ciclo celular in vitro de linfócitos suínos em uma maneira dependente da dose e do tempo, e a freqüência basal baixa de SCEs de linfócitos suínos independe da presença ou não de eritrócitos na cultur

Erythrocytes modulate cell cycle progression but not the baseline frequency of sister chromatid exchanges in pig lymphocytes