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Clinical and serological follow up examinations were performed on 203 persons, from three to twenty years of age, from the otolaryngology department of a hospital in the city of Rio de Janeiro, with no symptomatology suggesting toxoplasmosis, but suffering from chronic tonsillitis. According to results obtained during the first indirect immunofluorescence tests, the patients were divided into following groups: Group I (non-reactive IgG and IgM), 98 persons (48.3%); Group II (1:16 &#8804; IgG &#8804; 1:256 and non-reactive IgM), 74 persons (36.5%); Group III (IgM &#8805; 1:1024 and non-reactive IgM), 18 persons (8.8%), and Group IV (IgG and IgM reactive), 13 persons (6.4%). One to two years later, 131 (64.5%) of the 203 persons were reexamined by a second indirect immunofluorescence test. In the case of 66 persons (Group I) whose serum was non-reactive in the IgG and IgM classes during the first indirect immunofluorescence test, serum conversion was observed in aproximately 21.2%. in 65 individuals (49.6%), (Groups II, III and IV),with reactive serum in the IgG classes during the first indirect immunofluorescence test, the second reaction showed an increase in titres in 20% of the cases, a decrease in 67.7% of the cases, or no alterations in 12.3 of the cases. In the IgM class, all 131 sera were non-reactive at 116 dilution the second immunofluorescence test, including the 13 cases that had previously been reactive in the immunoglobulin class, Symptomatology suggesting toxoplasmosis was only observed in one case during the second testing, this patient's principal physical sign being hypertrophied lymph nodes. during this period, the Toxoplasma antibodies showed titres of IgG 1:32000 and non-reactive IgM, whilst one year previously, during the first test, these titres were IgG 1:1024 and IgM 1:64. Differences in the age, sex and skin coloring of patients were not statistically significant as regards alterations in the indirect immunofluorescence test titres. Foi feito o acompanhamento sorológico e clínico de 203 indivíduos de 3 a 20 anos de idade, provenientesde serviços de otorrinolaringologia na cidade do Rio de Janeiro, sem sintomatologia sugestiva de toxoplasmose, mas portadores de amigdalite crônica. Os pacientes, de acordo com os resultados da 1ª RIFi, foram divididos nos seguintesgrupos: Grupo I (IgG e IgM não reagente), 98 indivíduos (48.3%); Grupo II (1:16 IgG &#8804; 1:256 e IgM não reagente), 74 indivíduos (36,5%); Grupo III (IgG &#8805; 1:1024 e IgM não reagente), 18 indivíduos (8,8%) e Grupo IV (IgG e IgM reagentes), 13 indivíduos (6,4%). Um a dois anos após, 131 (64,5%) dos 203 indivíduos foram reexaminados para uma segunda RIFi. Dentre 66 deles (Grupo I), que eram soro não reagentes nas classes IgG e IgM na primeira, RIFI, observou-se soro conversão nas classes IgG em cerca de 21,2%. Em 65 (49,6%) indivíduos (Grupos II, III e IV),que eram soro reagentes nas classes IgG na primeira RIFI, observou-se que na segunda reação, os títulos variaram de modo ascendente em 20% dos casos, descendente em 67,7% dos casos ou não variaram em 12,3% dos casos. Na classe IgM todos os 131 soros apresentaram-se não reagentes à diluição de 1:16 na segunda RIFi, inclusive os 13 casos anteriormente reagentes nesta classe de imunoglobinas. Apenas em um caso observou-se sintomatologia sugestiva de toxoplasmose na ocasião da segunda coleta, sendo que o paciente apresentava, principalmente, gânglios hipertrofiados. Os títulos de anticorpos para toxoplasmose nesta época foram IgG 1:32000 e IgM não reagente enquanto que na primeira coleta um ano antes eram IgG 1:1024 e IgM 1:64. Quanto à idade, sexo e cor as diferenças nos títulos da RIFI foram estatisticamente não significantes

memórias do instituto oswaldo cruz

Indirect immunofluorescence(IgG and IgM) tests for toxoplasmosis on 203 persons, with no symptomatology suggesting the disease, located in the city of Rio de Janeiro. Serological follow up one to two years later.