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EnglishAmong the Cronobacter genus, Cronobacter sakazakii is the most common species posing a severe health risk for newborns, infants and children. Some infant formulas, cereal-based foods, and food production environments may be the potential reservoirs of C. sakazakii. This pathogen possesses different virulence factors encoded by different virulence genes. Therefore, characterizing these genes is important for distinguishing pathogenic strains from nonpathogenic ones. The objective of this study was to characterize some virulence genes [OmpA, OmpX, zpx, and Cpa] by real-time polymerase chain reaction (PCR) in C. sakazakii isolates from a total of 120 samples (20 each of milk powder, starch, rice flour, semolina, infant formula and dust samples from food production environments). Overall, 13 isolates (7 from milk powder, 2 rice flour, 1 semolina, and 3 dust) were cultured, identified by bioMerieux API® 20E test kit, and then subjected to real-time PCR application for screening the target virulence-associated genes. Our results showed that all of 13 isolates were positive for the virulence genes OmpA, OmpX, zpx, and Cpa. In summary, our study revealed that some of the analyzed foods and environmental samples were contaminated with pathogenic C. sakazakii with its virulence-associated markers, far above the allowable limit; and therefore, this level of contamination may pose a severe health threat for newborns, infants, and children. Key words: C. sakazakii; dust; food; health; virulence genes. portuguesDentre o genero Cronobacter, Cronobacter sakazakii e a especie mais comum que representa um grave risco para a saude dos recem-nascidos, bebes e criancas. Algumas formulas infantis paracriancas, alimentos a base de cereais e locais de producao de alimentos, foram considerados como potenciais locais de contaminacao de C. sakazakii. Este patogeno possui diferentes agentes de virulencia codificados por diferentes genes de virulencia. Portanto, a caracterizacao dos genes e importante para distinguir as cepas patogenicas das nao patogenicas. O objetivo deste estudo foi caracterizar os diferentes genes de virulencia [OmpA, OmpX, zpx, and Cpa] em C. sakazakii isolados em um total de 120 amostras (20 de cada uma delas - leite em po, amido, farinha de arroz, semola, comida para bebes e amostras de poeira provenientes dos ambientes de producao de alimentos) por reacao em cadeia da polimerase em tempo real (RT-PCR). No total, foram cultivadas 13 estirpes (7 de leite em po, 2 de farinha de arroz, 1 de semola e 3 poeiras) e depois identificadas pelo kit de teste bioMerieux API® 20E. As estirpes identificadas foram submetidas ao PCR em tempo real para caracterizar os genes alvo associados a virulencia. Os resultados mostraram que todos os genes C. sakazakii isolados eram patogenicos e positivos as OmpA, OmpX, zpx e Cpa com um padrao de coexistencia. Em resumo, o nosso estudo revelou que os alimentos e os ambientes de producao de alimentos analisados ​​constituiam uma ameaca a saude dos recem-nascidos, bebes e criancas devido a contaminacao por C. sakazakii patogenico como marcadores associados a virulencia. Palavras-chave: C. sakazakii; Poeira; alimentacao; genes de virulencia.

Occurrence and molecular characterization of different virulence-associated genes of Cronobacter sakazakii isolates from some foods and dust samples