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EnglishDespite the known importance of Clostridium perfringens as an enteropathogen in small ruminants, little is known about the role of its additional virulence factors or the frequency of the various C. perfringens genotypes in healthy goats; this complicates the laboratory diagnosis of the infections caused by this microorganism. In light of this, the aim of the present study was to isolate and genotype C. perfringens from stool samples from healthy goats in Brazil. Stool samples from 250 apparently healthy adult goats from 17 different herds in Minas Gerais, Brazil were collected, and isolation and genotyping of C. perfringens was performed. C. perfringens type A was isolated from 189 (75.6%) goats, whereas C. perfringens types C and D were each detected in one goat (0.4%). All isolates were negative for enterotoxin-, NetB-, NetE-, and NetF-encoding genes. These results confirmed C. perfringens type A as part of the microbiota in these animals, and they suggested that C. perfringens type C and D are rarely isolated from healthy goats. Key words: Clostridium perfringens type D; microbiota; epsilon; beta portuguesApesar da reconhecida importância de Clostridium perfringens como enteropatogeno de pequenos ruminantes, pouco se sabe sobre a frequencia dos genotipos ou do papel de fatores de virulencia adicionais de C. perfringens em cabras saudaveis, dificultando o diagnostico laboratorial da infeccao causada por esse micro-organismo. Dessa forma, o presente estudo teve como objetivo caracterizar C. perfringens de amostras de fezes de cabras adultas saudaveis. Amostras de fezes de 250 cabras saudaveis de 17 rebanhos diferentes em Minas Gerais, Brasil, foram submetidas ao isolamento e genotipagem de C. perfringens. C. perfringens tipo A foi isolado de 189 (75,6%) cabras, enquanto C. perfringens tipos C e D foram detectados em um animal (0.4%) cada. Todos os isolados foram negativos para os genes codificadores das toxinas NetB, NetE, NetF e enterotoxina. Os resultados apresentados confirmam C. perfringens tipo A como parte da microbiota de cabras saudaveis e sugere que C. perfringens tipos C e D sao raramente encontrados em caprinos saudaveis. Palavras-chave: Clostridium perfringens tipo D; microbiota; epsilon; beta

Isolation and genotyping of Clostridium perfringens from goats in Minas Gerais, Brazil