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The genetic information of ancient  Paragonimus westermani , the oriental lung fluke infecting over 20 million people worldwide, has not been thoroughly investigated thus far. We analysed genetic markers (COI and ITS2) of  P. westermani  from coprolite specimens (n = 6) obtained from 15th to 18th century Korean mummies. Our results indicated that all  P. westermani  sequences were generally distinct from the other species of the genus  Paragonimus . The sequences were clustered into three groups: Group I for East Asia; Group II for South and Southeast Asia; and Group III for India and Sri Lanka. In this study, we found that ancient  P. westermani  sequences in Korea belong to Group I, adding invaluable information to the existing knowledge of  Paragonimus  paleogenetics.

Analysis of COI and ITS2 regions of DNA obtained from Paragonimus westermani eggs in ancient coprolites on Joseon dynasty mummies