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BACKGROUND   TcP21 is a ubiquitous secreted protein of  Trypanosoma cruzi  and its recombinant form (rP21) promotes parasite cell invasion and acts as a phagocytosis inducer by activating actin polymerisation in the host cell.    OBJECTIVE   Our goal was to evaluate if the additional supplementation of rP21 during a prime/boost/challenge scheme with  T. cruzi  TCC attenuated parasites could modify the well-known protective behavior conferred by these parasites.    METHODS   The humoral immune response was evaluated through the assessment of total anti- T. cruzi  antibodies as well as IgG subtypes. IFN-γ, TNF-α and IL-10 were measured in supernatants of splenic cells stimulated with total parasite homogenate or rP21.    FINDINGS   Our results demonstrated that, when comparing TCC+rP21  vs.  TCC vaccinated animals, the levels of IFN-γ were significantly higher in the former group, while the levels of IL-10 and TNF-α were significantly lower. Further, the measurement of parasite load after lethal challenge showed an exacerbated infection and parasite load in heart and skeletal muscle after pre-treatment with rP21, suggesting the important role of this protein during parasite natural invasion process.    MAIN CONCLUSION   Our results demonstrated that rP21 may have adjuvant capacity able to modify the cytokine immune profile elicited by attenuated parasites.

Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by Trypanosoma cruzi attenuated parasites