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Several studies have described the use of non-invasive collection methods, mostly based on the detection of parasite DNA, for diagnosis. However, no  Leishmania  specimens have been isolated from saliva. Here, we report the first isolation of  Leishmania braziliensis  from the saliva of humans with cutaneous leishmaniasis but without lesions on their mucosa. The isolates were obtained from salivary fluid inoculated in hamsters and were tested by multilocus enzyme electrophoresis. Seven samples from 43 patients suspected of having the disease were identified for  in vivo  culture. These findings suggest that saliva is a clinical sample that allows the isolation of  Leishmania  sp.

Leishmania (Viannia) braziliensis isolated from the saliva of patients in a cutaneous leishmaniasis-endemic area of northeastern Brazil