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Chemical composition of pollen is highly varied depending on the plant species from which it comes and has been the subject of numerous comparative studies. The aim of this study was to determine chemical composition and antioxidant activity of walnut pollen samples and compare them with those of bee pollen. Total phenols content, total flavonoids content, antioxidant activity and mineral composition were studied using walnut pollen samples from three walnut genotypes cultivated in Romania. Total phenols content was determined by colorimetric assay and their amount varied between 10.8 and 17.64 mgGAE/g per genotype. Determination of flavonoids was done by aluminium nitrate colorimetric method and total flavonoid contents in walnut pollen ranged from 7.32 to 7.95 mgQE/g. The antioxidant capacity of pollen extracts was assessed through the scavenging effects on DPPH and a concentration-dependent genotype, and it varied between 13.78 and 15.04 mg Trolox/g. In terms of mineral composition, walnut pollen appears to be a good source of potassium (859.14 mg/100 g), magnesium (263.77 mg/100 g), calcium (71.72 mg/100 g), iron (27.19 mg/100 g), sodium (10.52 mg/100 g), zinc (5.69 mg/100 g), manganese (3.98 mg/100 g), copper (1.28 mg/100 g), chromium (0.39 mg/100 g) and selenium (0.036 mg/100 g). The results obtained indicate that walnut pollen is an important source of total phenols showing antioxidant properties and mineral composition that could be beneficial to human health.

Chemical Composition and Antioxidant Activity of Walnut Pollen Samples