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AbstrakL-asparaginase (EC 3.5.1.1) adalah enzim yang menghidrolisis asam amino L-asparagin menjadi amonia dan asam aspartat. Enzim ini mempunyai manfaat utama dalam bidang farmasi dan industri pangan. Enzim L-asparaginase tersebar secara luas pada mikroorganisme. Mikroorganisme yang mempunyai potensi menghasilkan enzim ini adalah mikroorganisme endofit dari tumbuhan mangrove. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri endofit penghasil L-asparaginase dari tumbuhan mangrove Buta-buta (E. agallocha). Skrining dilakukan dengan menggunakan medium selektif untuk mendapatkan bakteri penghasil enzim L-asparaginase. Identifikasi molekuler dilakukan dengan menggunakan analisis filogenetik berdasarkan data sekuen 16S rDNA. Dari hasil penelitian ini didapatkan lima isolat bakteri endofit penghasil enzim L-asparaginase, di mana isolat penghasil L-asparaginase tertinggi diidentifikasi secara molekuler. Hasil identifikasi filogenetik molekuler menunjukkan bahwa isolat kode D.104 teridentifikasi sebagai Enterobacter cloacae. Molecular Identification of L-asparaginase-Producing Endophytic Bacteria Isolated from Mangrove Buta-Buta (Excoecaria agallocha)AbstractL-asparaginase (EC 3.5.1.1) is an enzyme which hydrolyze amino acid L-asparagine to aspartate and ammonia. Two main applications of this enzyme are in the pharmaceutical and food industries. The enzyme is widely distributed on microorganism. A potential source of L-asparaginase-producing bacteria is an endophytic bacteria from mangrove plant. This study aimed to isolate and identify L-asparaginase-producing endophytic bacteria from a mangrove plant, E. agallocha (Buta-buta). A screening was carried out using a selective medium to obtain the L-asparaginase enzyme producing bacteria. Molecular identification was carried out using phylogenetic analysis based on 16S rDNA sequence data. In this study, five isolates of the L-asparaginase-producing endophytic bacteria were obtained. The molecular phylogenetic identification showed that the highest L-asparaginase-producing bacterial isolate (code D.104) was identified as Enterobacter cloacae.

Identifikasi Molekuler Bakteri Endofit Penghasil L-asparaginase yang Diisolasi dari Mangrove Buta-Buta (Excoecaria agallocha)