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Changes of environmental conditions in urban areas restrict the growth of many species (Gillner et al., 2013). Growth and vitality are considerably influenced by microclimatic conditions, especially changes in air humidity and air temperature caused by reradiation effects, higher surface temperatures, and higher winds pells (tunel effects) in streets (Sieghardt et al., 2005; Huang et al., 2008). Lack and irregular distribution of water cause periods of drought. Drought is the most important environmental stress, severely impairing plant growth and development (Anjum et al., 2011). It is known that drought significantly influences photosynthesis by changes in metabolism and regulation of stomatal conductance (Bota et al., 2004). Stomatal responses to changes in environment are assential for the plants acclimation to environmental conditions (Heterington and Woodward, 2003; Berry et al., 2010). Stomatal closure is a protective strategy against waterloss and xylem hydraulic failure (Choat et al., 2007; Chen et al., 2010). In relation to plant strategy of stomatal control used to eliminate consequences of water losses, there are two known types of plants. Isohydric plants under drought conditions close their stomata gradually to reduce gas exchange and waterloss (Kumagai and Porporato, 2012; Sade et al., 2012). Anisohydric plants tolerate a decline in water potential by keeping stomata open to enable continuous gas exchange within certain levels of water stress (Tardieu and Simonneau, 1998). Stomatal closure is generally considered a protective mechanism against drought stress (Tyree et al., 1998). Stomata have two main functions in plants. They participate in photosynthesis course and optimise water balance. Stomata open after sunrise (when there is light for photosynthesis) and close after sunset (Procházka et al., 1998). Stomata play key role in retaining water in plant. Plants are able create tolerance against water deficit by creation of tolerance to conditions of water deficit or by prevention of water loose (Bray, 2001). Transpiration is related to water content in leaves. Relative water content (RWC) indicates metabolic changes in plants. RWC represents the total amount of water needed by a plant at full saturation. The RWC expresses the water content in per cent at a given time as related to the water content at full turgor (González and González Vilar, 2001). Decrease of the RWC is accompanied with changes in physiological functions of plants, synthesis of growth and stock substances and metabolical changes. Relationship between the RWC, water content in soil and stomatal conductance could be an indicator of plants reactions to water stress. RESPonSE oF SToMATAL ConDUCTAnCE oF Acer cAmpestre L. To GRADUAL DRyInG oF THE SUBSTRATUM

Response of Stomatal Conductance of Acer campestre L. to Gradual Drying of the Substratum