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Simple, swift and sensitive method for Spectrophotometric determination of thiamine (VB1) in pharmaceutical tablets has been described. The proposed method is based on the reaction of thiamine (VB1) and 7-Chloro-4-nitrobenzoxadiazole (NBD-Cl) at alkaline medium (pH 10.5) to develop a deep brown adduct that bears maximum absorption at 434 nm. Beer’s law is obeyed in the concentration range 5-35 µg/ml of thiamine at the selected wavelength. Under optimized reaction conditions, the linear regression coefficients were a=0.033, b=-0.009, and r2=0.999 calculated for the general equation of the calibration curve (y = ax + b).  The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.667µg/ml and 2.020µg/ml respectively. The method has been successfully applied to the determination of thiamine (VB1) in pharmaceutical formulations, and can be used as an alternative of the existing sophisticated method used in drug laboratories and factories.

journal of analytical and pharmaceutical research

Application of Spectrophotometric Methods for the Determination of Thiamine (VB1) in Pharmaceutical Formulations Using 7-Chloro-4-Nitrobenzoxadiazole (NBD-Cl)