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A new treatment for inducing superovulation in adult rats was examined. Virgin Wistar-Imamichi rats at 11 weeks of age were pretreated with PMSG on the metestrus day. Then, the animals were anesthetized with sodium pentobarbital on the expected estrus day. The maximum number of ova shed in the animals thus treated was 100 with a combination of 40 I. U. of PMSG and hCG. If the treatment with sodium pentobarbital was omitted, the maximum number remained at 43 with different combinations of PMSG and hCG dosages.

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Effect of Sodium Pentobarbital on the Number of Ova Shed in Superovulation Treated Adult Rats: Relationship between the Number of Ova Shed and Dose of GTHs