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Efficiency of sterilization was shown to vary according to the type of autoclave, and this would be taken into consideration for preparing sterilized diet and water for laboratory animals. It seems necessary to sterilize diet and water for 60 and 150 minutes, respectively, at 120 degrees C using the autoclave in the Laboratory Animal Center of the Nippon Veterinary and Zootechnical Collge.

Sterilization of Diets and Drinking Water in the Autoclave in the Laboratory Animal Center, Nippon Veterinary and Zootechnical College