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Bone cells constitutively release ATP into the extracellular environment where it acts locally via P2 receptors to regulate bone cell function. Whilst P2Y 2 receptor stimulation regulates bone mineralisation, the functional effects of this receptor in osteoclasts remain unknown. This investigation used the P2Y 2 receptor knockout ( P2Y 2 R -  /  -  ) mouse model to investigate the role of this receptor in bone. MicroCT analysis of P2Y 2 R  -/- mice demonstrated age-related increases in trabecular bone volume (≤48%), number (≤30%) and thickness (≤17%). In vitro P2Y 2 R  -/- osteoblasts displayed a 3-fold increase in bone formation and alkaline phosphatase activity, whilst P2Y 2 R  -/- osteoclasts exhibited a 65% reduction in resorptive activity. Serum cross-linked C-telopeptide levels (CTX, resorption marker) were also decreased (≤35%). The resorption defect in P2Y 2 R  -/- osteoclasts was rescued by the addition of exogenous ATP, suggesting that an ATP deficit could be a key factor in the reduced function of these cells. In agreement, we found that basal ATP release was reduced up to 53% in P2Y 2 R  -/- osteoclasts. The P2Y 2 receptor agonists, UTP and 2-thioUTP, increased osteoclast activity and ATP release in wild-type but not in P2Y 2 R  -/- cells. This indicates that the P2Y 2 receptor may regulate osteoclast function indirectly by promoting ATP release. UTP and 2-thioUTP also stimulate ATP release from osteoblasts suggesting that the P2Y 2 receptor exerts a similar function in these cells. Taken together, our findings are consistent with the notion that the primary action of P2Y 2 receptor signalling in bone is to regulate extracellular ATP levels.

Activation of the P2Y2 receptor regulates bone cell function by enhancing ATP release