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Methods Retrospective study of patients with diagnosis of differentiated thyroid cancer, who underwent total thyroidectomy during 2002 and 2013 (n:256) in our hospital. Patients with pathological diagnosis of PTC, whose BRAF V600E mutation status was known (n:170) and who underwent lymphadenectomy (n:118) as well, were selected. LNM presence was correlated with BRAF V600E mutation status (presence of BRAF V600E mutation vs wild type) and with other clinico-pathological factors (age, initial tumor size, gender, etc). A multivariate analysis was performed to assess independent factors related to LNM. DNA was extracted from paraffin-embebbed tissues section and V600E mutations were detected by HRM followed by sequencing confirmation.

Prediction of lymph node metastasis in papillary thyroid cancer by preoperative BRAF analysis, is it useful?