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The aim of our three-year study was to determine the infl uence of sewage sludge and a consortium of aerobic microorganisms (BAF) on the dynamics of the development and activity of proteolytic and ammonifying bacteria, soil plant analysis development (SPAD), and the ratio of the leaf assimilation area in willows. Four experimental variants were used in the study: control, soil with sewage sludge, soil with sewage sludge and the BAF inoculant, and soil with the BAF inoculant. The experiment was conducted on the Start willow cultivar. In each year of our research (2013-15), soil samples for microbiological and chemical analyses were collected three times – in spring, summer, and autumn (in May, July, and September). The research involved the application of the Koch plate method to measure the count of proteolytic and ammonifying bacteria, and spectrophotometry to determine protease and urease activity. The green seeker index (SPAD) was measured by means of non-destructive methods with a SPAD index. The ratio between the willow leaf assimilation area and leaf area (LAI) was measured by means of the SunScan Canopy Analysis System. The content of chlorophyll a and b was analyzed by means of dimethyl sulfoxide

polish journal of environmental studies

The Influence of Sewage Sludge and a Consortium of Aerobic Microorganisms Added to the Soil under a Willow Plantation on the Biological Indicators of Transformation of Organic Nitrogen Compounds