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In this study, we conducted a phytochemical screening followed by evaluation of the antimicrobial and antioxidant activity of essential oils and polyphenols of Citrus aurantium. In parallel, we evaluated the insecticide effect of polyphenolic extract of Citrus aurantium L. on the larvae of Galleria mellonela, for this, we evaluated the mortality rate, TL50 and DL50. The phytochemical screening has allowed us to highlight the presence of secondary metabolites in the leaves and bark of Citrus, like saponins, mucilage, and flavonoids with significant amounts. Observations on the antibacterial activity show that Staphylococcus aureus is the most sensitive strain, followed by Escherichia coli, the polar extracts proved devoid of any antifungal activity against Saccharomyces cerevisiae, and Aspergillus sp. Two phytopathogenic bacteria were treated with polyphenols of Citrus aurantium ; Pseudomonas savastanoi and Pectobacterium spedonicus. The test of antibacterial activity showed a growth inhibition of these two phyopathogènes bacteria. The evaluation of the antioxidant, revealed an important activity. Concerning the insecticidal effect of the polyphenols of the bitter orange leaves on the larvae of the wax moth Galleria mellonela, very significant results are achieved with 100% mortality recorded 24 hours after treatment at the high dose (30μl / ml). Keywords— Citrus aurantium L., characterization, essential oil, polyphenols, activity. Oulebsir-Mohandkaci Hakima1 Laboratory of Valorization and Conservation of Biological Resources, Department of Biology, Faculty of Sciences, University M'hamed Bougara of Boumerdes, BP35000, Algeria Ait Kaki Sabrina2 Laboratory of Soft Technology, Valorization, Physicochemical of Biological Material and Biodiversity. University M’hamed Bougara of Boumerdes. 35000, Algeria. Behidj Nassima3 Laboratory of Soft Technology, Valorization, Physicochemical of Biological Material and Biodiversity. University M’hamed Bougara of Boumerdes. 35000, Algeria.

international journal of advances in chemical engineering and biological sciences

Phytochemical Study and Evaluation of Antimicrobial, Antioxidant and Insecticidal Activity of Essential Oils and Polyphenols of Bitter Orange (Citrus Aurantium L.)