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We used a combination of Extended X-ray Absorption Fine Structure (EXAFS) spectroscopy, Transmission Electron Microscopy (TEM) and Energy-Dispersive X-ray (EDX) analysis to conduct molecular scale studies on U(VI) interaction with three recently described eco-types of  Acidithiobacillus ferrooxidans . On the basis of the information obtained by using these methods, we concluded that uranyl phosphate complexes were formed by the cells of the three eco-types studied. The uranium accumulated by  A. ferrooxidans  cells was located mainly within the extracellular polysaccharides, and on the cell wall. Smaller amounts were also observed in the cytoplasm.

radiochimica acta

Characterization of U(VI)- Acidithiobacillus ferrooxidans  complexes using EXAFS, transmission electron microscopy, and energy-dispersive X-ray analysis