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A systematic chemical study of the secondary metabolites of the marine fungus,  Penicillium chrysogenum  (No. Y20-2), led to the isolation of 21 compounds, one of which is new (compound  3 ). The structures of the 21 compounds were determined by conducting extensive analysis of the spectroscopic data. The pro-angiogenic activity of each compound was evaluated using a zebrafish model. The results showed that compounds  7 ,  9 ,  16 , and  17  had strong and dose-dependent pro-angiogenic effects, with compound  16  demonstrating the strongest pro-angiogenic activity, compounds  6 ,  12 ,  14 , and  18  showing moderate activity, and compounds  8 ,  13 , and  19  exhibiting relatively weak activity.

Secondary metabolites from the marine-derived fungus Penicillium chrysogenum Y20-2, and their pro-angiogenic activity