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Phytochemical investigation of the stem bark extract of  Crossopteryx febrifuga  resulted in the isolation of epimeric mixtures of 3 β -urs-12,20(30)-diene-27,28-dioic acid and 18-epi-3 β -urs-12,20(30)-diene-27,28-dioic acid ( 1 ), as well as: 3 β -D-glucopyranosylurs-12,20(30)-diene-27,28-dioic acid and 18-epi-3 β -D-glucopyranosylurs-12,20(30)-diene-27,28-dioic acid ( 2 ), together with some known compounds such as the monoglyceride of palmitic acid, as well as  β -sitosterol and its glucoside. The structures of the isolated compounds were determined by application of spectroscopic methods. The MeOH extract and compounds  1  and  2  were examined for antimicrobial activity in  in vitro  assays against bacteria ( Enterobacter aerogenes  ATCC13048,  Escherichia coli  ATCC8739,  Klebsiella pneumoniae  ATCC11296,  Staphylococcus aureus ) and fungi ( Candida parapsilosis ,  Candida albicans  ATCC 9002 and  Cryptococcus neoformans  IP 90526). The tested samples showed selective activities. The antibacterial and antifungal activities of compound  2  (MIC=8–64 μg/mL) were in some cases equal to or even higher than those of the respective reference drugs chloramphenicol (MIC=16– 64 μg/mL) and nystatin (MIC=128–256 μg/mL).

Antimicrobial triterpenes from the stem bark of Crossopteryx febrifuga