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Biosurfactants are surface active substances which reduce interfacial tension and are produced or excreted at the microbial cell surface. We evaluated the biosurfactant production by Aspergillus fumigatus and Phialemonium sp. in solid state processes using fixed-bed column reactors. We evaluated two media, rice husks alone (simple support) and rice husks plus defatted rice bran (complex support), both enriched with either soy oil or diesel oil. The highest water-in-oil emulsifying activity (EA w/o ) obtained was 7.36 EU g -1  produced by A. fumigatus growing on complex support enriched with soy oil and supplied with air at a rate of 60 mL g -1  h -1 , while Phialemonium sp. had a maximum production of 6.11 EU g -1  using the simple support with diesel oil and an aeration rate of 120 mL g -1  h -1 . The highest oilin- water emulsifying activity (EA o/w ) was 12.21 EU g -1  produced by Phialemonium sp. on the complex support enriched with diesel oil and at an aeration rate of 60 mL g -1  h -1 , while A. fumigatus produced a maximum EA o/w  of 10.98 EU g -1  when growing on the complex support with no additional carbon source and an aeration rate of 60 mL g -1  h -1 .

Solid State Biosurfactant Production in a Fixed-Bed Column Bioreactor